| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilson, Christian (12)
|
NITWST |
28.33 |
27.84 |
-0.49 |
| 2 |
Haak, Miles S (12)
|
NITWST |
29.84 |
29.08 |
-0.76 |
| 3 |
Mallory, John B (11)
|
NIUN |
31.21 |
31.31 |
0.10 |
| 4 |
Bembenista, Owen A (11)
|
NITWST |
32.00 |
31.61 |
-0.39 |
| 5 |
Huber, Caleb J (12)
|
NIUN-T |
32.42 |
32.26 |
-0.16 |
| 6 |
Bruzgul, Jaxon R (11)
|
NITWST |
32.25 |
32.40 |
0.15 |
| 7 |
Adams, Marshall B (11)
|
NITWST |
33.64 |
32.80 |
-0.84 |
| 8 |
Carney, Deaglan C (11)
|
NIEAST |
34.30 |
32.93 |
-1.37 |
| 9 |
Vallely, McKean (11)
|
NIEAST |
33.67 |
33.13 |
-0.54 |
| 10 |
Kjellstrom, Anders J (11)
|
NITWST |
33.39 |
33.60 |
0.21 |
| 11 |
Seifert, Jacob D (12)
|
NITWST |
33.59 |
34.26 |
0.67 |
| 12 |
Knowles, Brody C (12)
|
NIPVSC |
34.77 |
34.86 |
0.09 |
| 13 |
Kitson, Jayson R (12)
|
NIEAST |
34.84 |
34.94 |
0.10 |
| 14 |
Sitarek, Matthew (11)
|
NITWST |
34.60 |
35.72 |
1.12 |
| 15 |
Kirchberger, Luke A (11)
|
NIHAMB |
38.16 |
35.95 |
-2.21 |
| 16 |
Rignel, Dean P (12)
|
NIEAST |
36.63 |
38.97 |
2.34 |
| 17 |
Bartolotti, Harry D (11)
|
NITWST |
40.35 |
40.52 |
0.17 |
| 18 |
Pfaffenbach, Kadin L (12)
|
NIPVSC |
40.66 |
41.60 |
0.94 |
| 19 |
Rugg, Matthew T (12)
|
NIPVSC |
45.23 |
47.31 |
2.08 |